The court in Jharkhand on Apr 29 had stayed Singh's arrest till May 3.
The court had ordered him to file a regular bail plea in this regard.
A Bokaro court in Jharkhand on Apr 28 rejected Singh's anticipatory bail plea in the case.
Additional District and Sessions Judge (First Class) Bipin Bihari rejected Singh's petition.
On Apr 25, a Patna civil court in Bihar granted anticipatory bail to Singh in connection with his alleged 'Pakistan' remark.
A non-bailable warrant was issued on Apr 23 against Singh for his alleged 'Pakistan' remark.
The Election Commission of India (ECI) on Apr 22 banned Singh from campaigning in Bihar and Jharkhand after the BJP candidate triggered a controversy by saying that all opponents of the party's prime ministerial candidate Narendra Modi will be sent to Pakistan.
The commission has asked authorities in both the states not to allow his 'public meetings, public processions, public rallies, road shows, etc., by the district administration authorities of Bihar and Jharkhand'.
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